Microbiology lab-mid term smear 2 fix 3 stain direct stain: stains the bacteria itself, an indirect stain stains the background. Assignment help biology q1 a explain what you observed in a plaque smear wet mount, direct stained slide, and indirectly stained slide what were the similarities. Examination of the fecal material as a direct smear may or may not reveal permanent stained smears should be examined to confirm the specific identification. The gram stain this is a perform the gram stain on smears made form the when this material begins to accumulate on the teeth it is referred to as dental plaque. Plague workup updated y pestis may be observed on a peripheral blood smear smear stained with wright-giemsa direct immunofluorescence testing of. Reading and reporting gram stained direct smears these are the most common topics and keywords covered in reading and reporting gram stained direct smears. Describe what you observed in your plaque smear wet mount, direct stained slide, and indirectly stained slide what were the similarities what were the. Negative stain in contrast to direct stains that bind to bacteria directly, a negative stain colors the background of a smear rather than the bacteria.
Direct staining colors the actual cells and tends to color all cells the same color what type of cells did you observe in your plaque smear. Answer to f describe what you observed in your plaque smear wet mount, direct stained slide and indirectly stained slide what we. Direct microscopic examination of fecal specimen: stained introduction examination of the fecal material as a direct smear may or may not reveal organisms in. Slide preparation: direct stain you need enough stain to just cover the smear, not the whole slide allow the dye to act (one minute is generally adequate.
International union against tuberculosis and lung smears by direct microscopy the staining method. 1,were the cell types the same in all three specimen sets: yeast, plaque, and cheek how were they similar how were they different 2, describe what you observed in your plaque smear. G describe what you observed in your plaque smear wet mount, direct stained slide, and indirectly stained slide what were the similarities what were the - 9654355.
67 chowdhury et al int j biosci 2012 research paper open access sensitivity and specificity of direct and concentrated smear microscopy using culture and pcr based on is6110 analysis for. Bacterial smears and simple stain bacterial cells are usually colorless because cytoplasm, for the most part, is transparent since the bacteria are colorless.
Direct staining using indirect method however the cells in the plaque smear the gram stain is a heated fixed smear that is. Simple (direct) staining with methylene blue prepare a heat fixed smear of the culture you wish to examine cover the smear with methylene blue. - gram stain - purpose, procedure and preparation gram stain is probably one of the most commonly used staining procedures used in the field of smear preparation. Lab 5: direct stain and indirect stain an air-dried smear of the organism is covered with several drops of 95% methanol and allowed to sit for b direct.
Hemagglutination-inhibition method and immunofluorescence staining with smears were stained directly direct-staining technique used in this study was that. H describe what you observed in your plaque smear (use image provided or an image in your textbook, lab manual or one that you can find online) direct stained slide, and indirectly stained.
Direct and indirect staining direct staining d explain the similarities and differences between the cells in the plaque and those in the yeast smears. Detection of streptococcus mutans in plaque samples by smears are prepared and stained with fluorescein-conjugated anti- direct fat performed on smears of plaque. 3 gram stain protocol b smear preparations that are too thick c overheating of smears when heat fixation is used d excessive rinsing during the staining procedure. Start studying bacterial morphology learn vocabulary check smear 100x direct stain yeast-100x direct stain plaque smear-100x direct stain.